Hand-gate a sample

Hand-gate a sample when one measurement is unusual — a shifted population, a clump, a disturbance — and the series’ gate counts it wrongly, while the gate is right for the samples around it. The gate you draw applies to this sample only.

Where

Flow Cytometry › sample › Analyze sample

Who

ManagerAdmin

1. In Flow Cytometry, select the sample.

2. Select Analyze sample.

3. Draw the gate, the ACC region and the HNA split for this sample, as in Edit a gate.

4. Select Save gate.

5. Under What does this apply to?, leave This sample selected.

6. Select Save gate.

Result: the message Gate saved appears. The sample is recomputed at once: its tiles and plots show the new numbers, Gate reads Hand-gated with the badge This sample only, and the earlier numbers are in its Calculation history as the row below the current one.

Hand-gated with This sample only: this sample keeps its own gate when its series’ gate changes.

What hand-gated means

• The sample no longer follows its series. New versions of the series’ gate, and history recomputes, leave it alone — permanently, until you put it back.
• Nothing else changes: not the series, not the gate library, not other samples.
• The hand gate is not stored in the gate library.

Put a sample back on its series’ gate

1. Select the hand-gated sample.

2. Open its menu (⋮) and select Use the series’ gate.

Result: the message This sample now follows its series appears and the sample is recomputed with the gate its series follows — or, if the series follows none, with the gate its instrument sent.

You can do the same from the gate editor: open Analyze sample on the hand-gated sample and select Use the series’ gate at the bottom left.

Note

A hand gate is meant for exceptions. When more than a few samples of a series need one, the series’ gate is wrong: edit the gate instead, or apply it to history.

If it does not work

Message	What to do
Could not switch back to the series’ gate	Nothing changed. Reload the page and try again.
Use the series’ gate is missing	The sample is not hand-gated, or you do not have the Manager or Admin role.

Related

• How gating works
• Edit a gate
• Flow Cytometry — the calculation history